Jena qTOWER3 fluorescence quantitative gene amplification instrument (PCR instrument)

Jena Fluorescence Quantitative PCR Instrument from Germany is a technological leader in fluorescence quantitative PCR instruments. It has fast reaction speed, precise temperature control, high detection sensitivity, flexible configuration (1-6 channels can be freely combined), Chinese software, convenient operation, and can significantly improve the efficiency of detection and research work. It is a highly cost-effective experimental tool.

Jena Fluorescence Quantitative PCR Instrument from Germany is a technological leader in fluorescence quantitative PCR instruments. It has fast reaction speed, precise temperature control, high detection sensitivity, flexible configuration (1-6 channels can be freely combined), Chinese software, convenient operation, and can significantly improve the efficiency of detection and research work. It is a highly cost-effective experimental tool.

  Product Introduction

The new generation of fluorescence quantitative gene amplification instrument qTOWER3 series from Jena, Germany, is designed in an integrated manner. It uses four unique high-efficiency LED solid-state light sources as excitation light sources and collects fluorescence signals through a patented fiber optic scanning system, ensuring excellent and stable excitation and detection of commonly used fluorescence quantitative PCR dyes on the market. Flexible detection channel configuration, easy upgrade, and can achieve simultaneous detection of up to six target genes. Jena Company has a nearly thirty year history in the development and manufacturing of gene amplification instruments. The fast and durable gold-plated pure silver sample tank ensures a 96 well temperature uniformity of ± 0.15 ℃. The optional 12 column linear temperature gradient function allows users to quickly determine the optimal PCR annealing temperature and optimize reaction conditions, making it a powerful assistant for users to optimize PCR programs. It has an open reagent and consumable system suitable for standard 96 well PCR plates, 8-way tubes, or PCR single tubes.     

  Innovation points

1. Fast: The maximum heating rate can reach 8 ℃/s, and the maximum cooling rate is 6 ℃/s. It is currently the fastest quantitative PCR instrument on the market, which can significantly save experimental time and improve work efficiency;
2. Convenience: Free channel selection, budget saving, and enjoying the latest performance; Easy to use and operate, cumbersome to advertise;
3. Chinese software interface: In order to facilitate the use of more and more Chinese users, a Chinese software interface has been developed, allowing users to enjoy both high-quality results and an intuitive and convenient user experience;

  Product Features

1. Rapid reaction: Gold plated pure silver sample tank, heated by semiconductor Peltier, with a maximum heating rate of 8 ℃/s, good thermal conductivity, and corrosion resistance and oxidation resistance
2. Accurate temperature control: The temperature accuracy is ± 0.1 ℃, and the temperature uniformity of 96 wells is ± 0.15 ℃. Accurate temperature control brings stable and specific amplification effects
3. Linear temperature gradient: Unique 12 column linear temperature gradient function, which can help users quickly find the most accurate annealing temperature (suitable for qTOWER3 G)
4. Automatic hot cap: Adopting advanced automatic hot cap technology, the instrument automatically controls the height and pressure of the hot cap to ensure effective anti evaporation effect
5. Consumable opening: There are no special requirements for consumables, and users can freely choose 96-hole plates, 8-way pipes, or single pipes, which is convenient to use

   Unique optical detection system   

1. High intensity and wide spectrum excitation light source: Four high intensity and long lifespan LED solid-state light sources, blue, green, red, and white (RGBW), are used to ensure strong light intensity throughout the entire spectrum detection range, improve detection sensitivity, and avoid preheating during use. The light source does not generate heat, has a long service life, and is maintenance free for life
2. High sensitivity detector: the new channel photomultiplier tube detector (CPMT) is the most sensitive detector currently applied to the quantitative PCR instrument
3. Fast scanning detection: 8-channel fiber optic scanning detection is fast, even if six channels of 96 hole full board scanning are completed, it only takes 6 seconds
4. No need for optical path correction: Scanning detection can ensure that the signal excitation and detection optical path of each hole are completely consistent, without the need for optical path correction, so there is no need to occupy an additional correction channel, which can achieve true six channel gene detection. Of course, if users wish to use internal reference dyes for correction, it can also be achieved
5. Flexible detection channel configuration: Users can flexibly choose the number of channels and the detection filter groups for each channel according to their needs. There are 12 different wavelength filter groups available to configure as 1-6 channel detection systems, including FRET detection combinations for filter mismatch and filter groups dedicated to protein fusion curve analysis, covering the full spectrum from blue to red light. The channel number can be easily upgraded at any time to meet more future detection needs

    Practical and convenient operation analysis software   

The operation and analysis software qPCRsoft of the qTOWER3 fluorescence quantitative PCR instrument can meet various application requirements of fluorescence quantitative PCR, such as absolute quantification, double standard curve relative quantification, double Delta Ct relative quantification (combined with amplification efficiency analysis), melting curve, allele typing/SNP analysis, endpoint analysis, amplification efficiency calculation, and other analysis methods. It can provide experimental reports that meet MIQE standards, Implementing information-based management of experimental reports and traceability of experimental information is a powerful tool for studying gene expression analysis, pathogen quantitative detection, SNP genotyping, and negative/positive detection.

1. During the operation of the instrument, real-time dynamic display of amplification curve, Ct value and other result information can be achieved
2. Sample holes can be defined after running the program, saving experimental time
3. When running the program, other experimental data results can be opened simultaneously for review and analysis
4. According to the actual fluorescence signal strength, the signal strength can be optimized by adjusting the detection gain value
5. The software automatically calculates the baseline range and sets threshold lines, providing users with reliable and accurate data results
6. It has functions such as multi gene analysis and multi plate analysis, and can easily handle relatively complex analysis and detection of multiple target genes and sample plates
7. When conducting multiple gene testing, the optical compensation function can correct signal cross interference between different channels, providing more accurate detection results
8. Amplified plots, standard curves, melting curves, etc. can be directly copied or saved as files, and the data results can be directly exported to Excel for analysis and storage
9. Experimental program setting information and detection results can be directly printed as experimental reports
10. The results of the same batch of experiments can be grouped for independent data analysis, and can be divided into up to 12 groups
11. Can provide experimental reports that comply with MIQE standards, achieving information-based management of experimental reports and traceability of experimental information
12. Providing a Chinese software interface greatly facilitates the use of Chinese users
13. Program settings and operation can be directly carried out on the built-in 10 inch large touch screen of the instrument. During operation, the amplification curve and Ct value information can be displayed in real-time, and the results can be easily imported into the computer
14. The software can be installed on multiple computers without restrictions, without the need to pay for usage rights, and can be upgraded for free for life
15. Analysis of protein melting curve
A specialized protein filter group with excitation wavelength of 490nm and emission wavelength of 580nm is suitable for protein melting curve analysis using SYPRO Orange fluorescent dye labeling. It has unique advantages in detecting protein thermal stability, optimizing protein crystal ligands and buffer conditions, and analyzing protein interactions.

    High performance brings high-quality testing results    

The excellent temperature control system and highly sensitive optical detection system provide users with accurate and reliable detection results.

• Wide linear detection range

Amplify and detect a specific gene in human genome DNA. The number of gene copies is 10 orders of magnitude from 100 to 109. Through standard curve detection, the software automatically calculates its R2>0.999, and the amplification efficiency is 100%.

• Outstanding detection repeatability

The standard deviation SD of Ct values between 96 wells was 0.07 when amplifying a specific gene sequence of E. coli.

• Strong applicability of reaction system size

QTOWER3 has no special requirements for the size of the amplification reaction system. Stable and accurate results can be obtained with a reaction volume of 5-80 µ l, and the volume size has no effect on the detection results. The single pore reaction volumes are 5 µ l, 10 µ l, 20 µ l, 30 µ l, 40 µ l, 50 µ l, 60 µ l, 70 µ l, and 80 µ l, respectively